Cryopreservation of Gametes and Embryos in India
Infertility is categorized as a condition in which a female is unable to conceive a baby naturally or is unable to carry the pregnancy for the full term even after years of satisfactory and fertile sex life. The condition can be present in men as well as in women. There are two kinds of infertility i.e. primary and secondary. It can be caused owing to a number of physical, lifestyle and emotional factors such as genetic mutation, unwholesome lifestyle, and stress. The fertility or infertility treatments can be accomplished through a number of techniques such as counseling, medicines, surgery, and dietary changes among others. We offer Cryopreservation of Gametes and Embryos treatment for all infertile couples who have lost all hopes of conceiving. Best cryopreservation hospitals in India provide them a ray of optimism to experience the joy of parenthood in life.
What is Cryopreservation of Gametes and Embryos?
Cryopreservation hospitals in Delhi employ the best of doctors who have the ability to freeze and defrost with retention of viability. These hold the capability to offer flexibility in human infertility therapy when the gametes or embryos are handled in vitro as frozen tissues can be stored indefinitely in the liquid nitrogen at -196°C. Cryopreservation is often done when there are more embryos and eggs than is required for a single IVF cycle. The additional embryos and eggs are saved to be possibly used later during the later cycles. Cryopreservation of human gametes and embryos is a significant and extensively used method in most embryology laboratories.
Why is embryo cryopreservation done?
Plenty of reasons aid to the urge of men and women might be choosing the option of freezing and storing the embryos:
- Extra embryos preserving for later destroying doesn’t seem like a fitting option.
- IVF process if fails for the first time, can definitely be retraced and tried again.
- The couple is not required to undergo the whole IVF treatment procedure again.
- The man and woman can have a baby and can also use the embryo later to bring in a second child into the world.
- Embryos can be saved by women prior to the treatment for cancer etc. This will help eliminate her chances of getting pregnant in the future.
- A donor program can be carried out at ease with the help of preservation method.
- Donation for a noble cause like research will also help make further eye opening discoveries about the treatment.
How is embryo cryopreservation done?
We shall start from the very cellular level, a cell consist mostly of water. The prime concern when freezing embryos is the situation of ice formation that takes place in and between the cells. Ice crystals can hurt the cell wall, these can also harm the small structures that reside inside the cell.
The embryo must be by all means safeguarded during the whole freezing process. This is done utilizing all fluids of all called cryoprotective agents (CPAs). CPAs are like the “anti-freeze” for cells. Doctors incorporate two different methods that will help freeze and preserve embryos, the two methods are slow programmable freezing and vitrification.
In the slow freezing method, the embryos are slowly frozen into all different stages. The CPAs are all added up to the embryos to help enhance the strength over 10 to 20 minutes. The embryos are cooled over two hours slower in a machine that helps lower the temperature minute by minute. Once frozen, they are stored in liquid nitrogen at an unimaginable temperature of -321° Fahrenheit.
Vitrification on the other hand is a freezing technique that uses much higher strengths of CPAs. With this method, the doctor initially mix CPAs with the embryos. CPAs that are strong may also have the tendency to hurt the cell. To prevent this, the embryos are quickly placed into the liquid nitrogen. The process changes the whole thing into an almost-solid state, like the glass. In this state, ice is unable to be formed. When required, the embryos have been slowly thawed. They are drenched in special fluids to remove the CPAs. This also restores the cell’s normal water balance.
When is embryo cryopreservation done?
Embryos are generally frozen for one to six days post their preparation.
How long can embryos be stored?
Once embryos are frozen, they are stored and sealed in liquid nitrogen freezers. Embryos can be frozen in liquid nitrogen for quiet many years. It is not known how long embryos can be frozen safely and still be useful. Some countries have set time limits on how long embryos can be stored.
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