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Stem cell delivery method
The doctors in our affiliated hospitals use numerous injection approaches to deliver stem cells. Contingent on each patient’s particular condition, one or multiple approaches of delivery will be used during a standard treatment session so as to maximize safety and effectiveness. It is imperative to remember that our protocols always prioritize security first and the inoculations procedures provided are little invasive but still a very focused on delivering the stem cells as close as possible from the injury location. For more info regarding the diverse injection approaches, please have a look below.

Intravenous injection

The intravenous injection (IV) can be defined as the infusion of liquid elements straight into a vein. IVs permit healthcare specialists to administer fluids, blood products and medicines directly into a patient’s bloodstream through a small tube. This permits rapid absorption and precise control over the dosage of the element administered, which is important for a multiplicity of medical processes. As stem cells can be taken by the circulatory system to every portion of the body, they will wander to the site of injury or disease.
Infusion steps

  • 1. The IV tubing is being set up appropriately and two IV bags (saline solution and stem cells) are being suspended on a raised stand.
  • 2. The inoculation site (where the IV will be inserted) is being sanitized.
  • 3. The IV catheter (IV catheters are fixed over the needle used to puncture the vein) is removed from its sterile wrapping and the needle is then inserted into the vein.
  • 4. The protective cover is being removed from the end of the IV tubing and is prudently inserted into the catheter hub. A piece of tape is placed over the catheter hub to secure the IV.
  • 5. The nurse checks on the movement of fluid into the vein during the entire process which lasts 30 to 60 minutes. If the patient feels any discomposure during or after the process, the medical staff on-site should be notified instantaneously.
  • 6. Once the infusion is done, the nurse closes the ro
  • ller clamp to stop the stream of fluid. She places a clean piece of gauze over the IV site and applies subtle pressure as the catheter is drawn out.
  • 7. Patients are requested to press sterile cotton wool onto inoculation site for at least 5 minutes.

Retrobulbar Injection

Retrobulbar injections (RB) are generally used worldwide to offer local anesthesia in the retrobulbar zone (behind the eye). During our stem cell treatment procedure, this category of injection is used to deliver stem cells as close as possible from the optic nerve and/or retina so as to better target the site of injury.
Infusion Steps
The entire procedure is speedy and safe (as described below) and typically allows our patients to get more advantages from the treatment. Please note that retrobulbar inoculations will only be provided to patients older than 11 years old. The concluding decision will be made by the attending doctor upon admission at the hospital.
  • 1. The patient is requested to lie down. A disinfectant is then cautiously applied on the skin from the lower eyelid margin to the lower orbital rim, respectively.
  • 2. The patient is asked to look toward the opposite side of the eye being treated and look marginally up. If the injection is done for the right eye, the patient should look at the top left side and vice versa.
  • 3. A thin needle is then be inserted perpendicularly about 2 millimeters deep in the quadrant between the outside 1/3 and inside 2/3 of the lower orbital rim.
  • 4. The needle passes the equator of the eye sphere and is then directed towards the upper nasal site until it is about 3 centimeters in depth. Stem cells are then be vaccinated into the retrobulbar space.
  • 5. Once the inoculation is done, the needle is smoothly removed and the ocular globe is recurrently compressed with sterile bandages for a number of minutes. Usually, the whole procedure is completed in about 15 minutes and the inoculation itself is done in a couple of seconds.

Intrathecal Injection

The Intrathecal Administration comprises of an injection made into the spinal canal so as to access the cerebrospinal fluid (CSF) and by extension, the central nervous system. This kind of administration permits delivering the stem cells to the brain and spinal cord in an easier and more effective way. Before the injection, a lumbar puncture (LP) is implemented in the first place so as to extract a little quantity of CSF and swap it by the stem cells.
Infusion Steps

  • 1. The patient is asked to not eat after 10 PM the day before the process.
  • 2. Patients will be relocated into a surgical operating suite where the doctor supported by two nurses, will give the injection.
  • 3. Patients will be placed on their side with their backs near the verge of the table or bed. A nurse will then help patients in bending their knees towards their belly and stretching their head to the chest in a fetal position. This position aids to separate the vertebrae so that the needle can be put in more straightforwardly.
  • 4. The doctor will first scrutinize the patient’s lower back and mark the appropriate insertion site (between two lumbar vertebrae from L2 to L5).
  • 5. The region is then cleaned with an antiseptic and a local anesthetic is introduced beneath the skin to numb the zone where the needle will be inserted into the spinal canal.
  • 6. Once the needle is in the accurate position, the stylet from spinal needle is then withdrawn and about 2 ml of cerebral spinal fluid is gathered.
  • 7. The stem cells (around 1ml) and saline solution (around 1 ml) will then be administered respectively via the needle into the cerebral spinal fluid.
  • 8. The process is completed by withdrawing the needle with the reinserted stylet while giving pressure on the perforation site. Placement of the needle, accompanied by the infusion, is normally concluded in 20 minutes to hour.
  • 9. All patients are asked to lie flat for 4 to 6 hours after the process to sidestep any provisional side-effects such as headaches, nausea, fevers, vomiting and/or pains in the legs. These symptoms are believed to be an outcome the change in fluid volume within the spinal canal. Even lying flat, some patients may still develop this uneasiness. These may last for up to 48 hours. Patients should inform the doctors if they have a very severe headache, unbending neck, loss of sensation under the puncture zone, or any leakage from the injection zone.

Intramuscular Injection

An intramuscular (IM) injection is a shot of medication given into a muscle. Intramuscular injections of stem cells can help patients with muscular dystrophy to get better health gains. These injections are given straight into the muscles of the affected regions. Upon admission, the doctors will inspect the patient and decide how many stem cell packets should be vaccinated locally into the affected muscles. The delivery technique has also been applied to treat lower limb ischemia and diabetic foot.
Infusion steps

  • 1. The patient is requested to take the suitable posture to access the affected muscles effortlessly.
  • 2. The injection location is sanitized. Stem cells are enunciated by a syringe (There are 3 portions of a syringe: the needle to go into the muscle, the cask to hold the medicine, the plunger to get medication into and out of the syringe).
  • 3. The nurse moderately presses on and pulls the skin around the inoculation location so that it is marginally tight. The needle is inserted in the affected muscle and the stem cells are shot up slowly.
  • 4. Once the injection completes, the injection location is pressed with dry cotton and the needle is dragged out swiftly simultaneously. A piece of gauze is then sited at the injection location.
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