Step1: Umbilical Cord Collection and Initial Testings
It is a common practice in China for mothers to willingly donate the umbilical cord blood from the birth of their healthy full-term kids. Every mommy who enters a hospital in China to give birth is automatically verified for all major infections. Detailed family histories are also taken from each mother before donation. Viezec follows the People’s Republic of China strict regulation regarding blood donation and not less than five test rounds are being piloted by nationally experienced third-parties on potential donors for pathogen detection. Only females who have been fully screened and found to be totally healthy are permitted to donate their umbilical cord and umbilical cord blood.
Step 2: Second Round of Testing
The second step in the screening procedurehappens in laboratory once the collected sample have been verified negatively for communicable sicknesses, it is then shifted to our laboratories and officially becomes the property of ours. From this phase, we are responsible for each and every sample that is processed and offered to patients. The collected sample begins a second round of testing and while following donor’s requisite from the USFDA and AABB, we also carry out analysis for cytomegalovirus, exogenous virus (human T-cell leukemia virus, HTLV), micro-organisms (aerobic bacteria, anaerobic bacteria, fungi, etc.), survival rate, stem cell surface markers and stem cell biological characteristics (colony forming aptitude, differential capacity) in each step to guarantee the highest quality to its cell products.
Step 3: Separation
Procurementof the cord blood serum and preparing the stem cells for culturing is accomplished by splitting the desired constituents from the cord blood. The blood cells need to be removed from the blood serum and stem cells in order to not hinder with future injections. A centrifuge is used to segregate the serum and stem cells from the red cells and platelets. This is extremely refined and specialized separation process. After separation has been completed, the stem cells are removed for culture.
Step 4: Culturing
The stem cells derived from each umbilical cord are sowed into a flask filled with culture medium. This medium does not encompass any animal products (such as fetal calf serum) but it is boosted with cell growth factors. The flasks are placed in a sterilized, temperature and humidity controlled incubator. The stem cells are prolonged in the culture medium. Once the culturing is complete, the culture medium is washed away and isgathered.
Step 5: Storage
The cell products are then cryo-preserved and stowed at a consistent -196 degree Celsius temperature with automatic supplement of liquid nitrogen using the greatly advanced Thermogenesis BioArchive system. This technology allows for integrated control rate of freezing that completelysidestepsunexpected drops in temperature. Our cryo-preservation storage equipment is also armed with 24h real-time control system and in any case of power-failure or other emergency, a redundant goofproofpower supply ensures that normal operation is carried out. Our vacuum protection system can retain proper temperature for 20 days without supplement of liquid nitrogen.
Step 6: Packing and transport
All batches of stem cells go through final testing. During this procedure, they are also tested for quantity and quality. Once the stem cells are estimatedto be safe, every one unit is placed into either a sterile vial or a sterile IV bag for future transplantation. All products are individually categorized and tracked while in shipment. Our cell transplants come about two to three days a week. All of our cells are processed on an as-needed basis. All of our cell products are processed and kept fresh, giving us the peak stem cell viability count and the maximum effectiveness.
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